SEARCH:   Advanced

Blood, 26 February 2009, Vol. 113, No. 9, pp. 1992-2002. Prepublished online as a Blood First Edition Paper on December 5, 2008; DOI 10.1182/blood-2008-02-133751. This Article Full Text (PDF) All Versions of this Article: blood-2008-02-133751v1 113/9/1992    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, H. Articles by Berenson, J. R Search for Related Content PubMed PubMed Citation Articles by Chen, H. Articles by Berenson, J. R Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 8, 2008 Accepted November 6, 2008 Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: A novel mechanism of tumor-induced vasculogenesis Haiming Chen, Richard A Campbell, Yunchao Chang, Mingjie Li, Cathy S Wang, Jennifer Li, Eric Sanchez, Michael Share, Jeffrey Steinberg, Ariana Berenson, Dror Shalitin, Zhaohui Zeng, Dorina Gui, Pablo Perez-Pinera, Ronald J Berenson, Jonathan Said, Benjamin Bonavida, Thomas F Deuel, and James R Berenson* Institute for Myeloma & Bone Cancer Research, West Hollywood, CA, United States Department of Molecular and Experimental Medicine and Cell Biology, The Scripps Research Institute, La Jolla, CA, United States Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, United States Department of Pathology, University of California at Los Angeles, Los Angeles, CA, United States HemaQuest, Seattle, WA, United States Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA, United States * Corresponding author; email: jberenson{at}imbcr.org. Enhanced angiogenesis is a hallmark of cancer. Pleiotrophin (PTN) is an angiogenic factor that is produced by many different human cancers and stimulates tumor blood vessel formation when it is expressed in malignant cancer cells. Recent studies show that monocytes may give rise to vascular endothelium. In these studies, we show that PTN combined with macrophage colony stimulating factor (M-CSF) induces expression of vascular endothelial cell (VEC) genes in human monocyte cell lines and monocytes from human peripheral blood (PB). Monocytes induce VEC gene expression and develop tube-like structures when they are exposed to serum or cultured with bone marrow (BM) from patients with multiple myeloma (MM) that express PTN, effects specifically blocked with anti-PTN antibodies. When co-injected with human MM cells into SCID mice, green fluorescent protein-(GFP)-marked human monocytes were found incorporated into tumor blood vessels and expressed human VEC protein markers and genes that were blocked by anti-PTN antibody. Our results suggest that vasculogenesis in human MM may develop from tumoral production of PTN which orchestrates the transdifferentiation of monocytes into VECs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020