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Blood, 15 January 2009, Vol. 113, No. 3, pp. 604-611. Prepublished online as a Blood First Edition Paper on October 9, 2008; DOI 10.1182/blood-2008-02-136903. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-02-136903v1 113/3/604    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Al Tabaa, Y. Articles by Vendrell, J.-P. Search for Related Content PubMed PubMed Citation Articles by Al Tabaa, Y. Articles by Vendrell, J.-P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 1, 2008 Accepted September 15, 2008 Functional Epstein-Barr virus reservoir in plasma cells derived from infected peripheral blood memory B cells Yassine Al Tabaa, Edouard Tuaillon, Karine Bollore, Vincent Foulongne, Gael Petitjean, Jean-Marie Seigneurin, Christophe Duperray, Claude Desgranges, and Jean-Pierre Vendrell* Department of Virology, University Medical Center of Montpellier, Montpellier, France Department of Virology, University Medical Center of Grenoble, Grenoble, France INSERM U847, Montpellier, France Institut Cochin, Universite Rene Descartes, CNRS (UMR 8104), Paris, France * Corresponding author; email: jp-vendrell{at}chu-montpellier.fr. The Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes latency in resting memory B lymphocytes, and is involved in oncogenesis through poorly understood mechanisms. The EBV-lytic cycle is initiated during plasma cell differentiation by mRNAs transcripts encoded by BZLF1 which induce the synthesis of EBV proteins such as the immediate-early antigen ZEBRA and the late membrane antigen gp350. Therefore, we assessed the capacity of circulating EBV-infected B lymphocytes from healthy EBV-seropositive subjects to enter and complete the EBV-lytic cycle. Purified B lymphocytes were polyclonally stimulated and BZLF1- or gp350-secreting cells (BZLF1-SCs or gp350-SCs) were enumerated by ELISpot assays. The number of BZLF1-SCs ranged from 50 to 480/107 lymphocytes (median 80, 25th-75th percentiles 70-150) and gp350-SCs from 10 to 40/107 lymphocytes (median 17, 25th-75th percentiles 10-20). gp350-SCs represented only 7.7 to 28.6% of BZLF1-SCs (median 15%, 25th-75th percentiles 10.5-20%). This EBV functional reservoir was preferentially restricted to plasma cells derived from CD27+ IgD- memory B lymphocytes. In 9/13 subjects, EBV-DNA quantification in B cell culture supernatants gave evidence of the completion of EBV-lytic cycle. These results demonstrate that EBV-proteins can be secreted by EBV-infected B lymphocytes from healthy carriers, a majority generating an abortive EBV-lytic cycle and a minority completing the cycle. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. Brady, H. J. Whiteman, L. C. Spender, and P. J. Farrell Downregulation of RUNX1 by RUNX3 Requires the RUNX3 VWRPY Sequence and Is Essential for Epstein-Barr Virus-Driven B-Cell Proliferation J. Virol., July 1, 2009; 83(13): 6909 - 6916. [Abstract] [Full Text] [PDF]

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