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Blood, 15 August 2008, Vol. 112, No. 4, pp. 1078-1084. Prepublished online as a Blood First Edition Paper on June 2, 2008; DOI 10.1182/blood-2008-02-139402. This Article Full Text (PDF) All Versions of this Article: blood-2008-02-139402v1 112/4/1078    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Olsson, B. Articles by Wadenvik, H. Search for Related Content PubMed PubMed Citation Articles by Olsson, B. Articles by Wadenvik, H. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 14, 2008 Accepted May 12, 2008 Recruitment of T-cells into bone marrow of ITP patients possibly due to elevated expression VLA-4 and CX3CR1 Bob Olsson*, Borje Ridell, Lena Carlsson, Stefan Jacobsson, and Hans Wadenvik Department of Internal Medicine, University of Gothenburg, Gothenburg, Sweden Department of Pathology, University of Gothenburg, Gothenburg, Sweden Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg, Gothenburg, Sweden * Corresponding author; email: bob.olsson{at}medic.gu.se. In idiopathic thrombocytopenic purpura (ITP), platelets are destroyed in the spleen, liver and bone marrow (BM) by autoantibodies and cytotoxic T-cells. In a DNA microarray screen of peripheral blood T-cells, we found that VLA-4, CX3CR1 and CXCR4, involved in T-cell homing, had increased expression in ITP patients compared with controls. However, we only found increased protein expression of VLA-4 on T-cells from peripheral blood by flow cytometry. To address a possible recruitment of T-cells into the organs involved in platelet destruction, we analyzed T-cells in BM. In BM, T-cell surface expression of VLA-4 and CX3CR1 was increased in ITP patients compared with controls. Furthermore, the number of CD3+ T-cells in BM, but not in blood, was increased in ITP patients compared with controls. This finding was confirmed by immunohistochemistry of BM biopsies. The number of regulatory T-cells (CD4+/CD25bright) was decreased in the BM of ITP patients whereas Fas expression was increased. In conclusion, ITP is associated with accumulation and activation of T-cells in the BM. Recruitment of T-cells into the target organ, e.g. BM, is plausible and may be facilitated through increased VLA-4 and CX3CR1 expression. These molecules might serve as new treatment targets in ITP. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: ITP three R's: regulation, routing, rituximab John W. Semple Blood 2008 112: 927-928. [Full Text] [PDF] This article has been cited by other articles: D. B. Cines, J. B. Bussel, H. A. Liebman, and E. T. Luning Prak The ITP syndrome: pathogenic and clinical diversity Blood, June 25, 2009; 113(26): 6511 - 6521. [Abstract] [Full Text] [PDF] J. W. Semple Platelet antigen-induced regulation in ITP Blood, March 12, 2009; 113(11): 2373 - 2373. [Full Text] [PDF] Fresh Insights into Chronic Immune Thrombocytopenic Purpura Journal Watch Oncology and Hematology, September 23, 2008; 2008(923): 2 - 2. [Full Text]

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