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Blood, 1 January 2009, Vol. 113, No. 1, pp. 75-84. Prepublished online as a Blood First Edition Paper on September 25, 2008; DOI 10.1182/blood-2008-02-139865. This Article Full Text (PDF) Supplemental Table, Figures, and Videos All Versions of this Article: blood-2008-02-139865v1 113/1/75    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mittelbrunn, M. Articles by Sanchez-Madrid, F. Search for Related Content PubMed PubMed Citation Articles by Mittelbrunn, M. Articles by Sanchez-Madrid, F. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 19, 2008 Accepted September 2, 2008 Imaging of plasmacytoid dendritic cell interactions with T cells Maria Mittelbrunn, Gloria Martinez del Hoyo, Maria Lopez-Bravo, Noa B Martin-Cofreces, Alix Scholer, Stephanie Hugues, Luc Fetler, Sebastian Amigorena, Carlos Ardavin, and Francisco Sanchez-Madrid* Departamento de Biologia Vascular e Inflamacion, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain Departamento de Inmunologia y Oncologia, Centro Nacional de Biotecnologia, Madrid, Spain Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain INSERM UMR653, Immunite et Cancer, Institut Curie, Paris, France CNRS UMR 168, Laboratoire de Physico-Chimie Curie, Institut Curie, Paris, France * Corresponding author; email: fsanchez.hlpr{at}salud.madrid.org. Plasmacytoid dendritic cells (pDCs) efficiently produce type I interferon and also participate in adaptive immune responses, although the molecular interactions between pDCs and antigen-specific T cells remain unknown. This study examines immune synapse (IS) formation between murine pDCs and CD4+ T cells. Mature pDCs formed canonical ISs, involving relocation to the contact site of the microtubule-organizing center (MTOC), F-actin, PKC- and pVav, and activation of early signaling molecules in T cells. However, immature pDCs were less efficient at forming conjugates with T cells and inducing IS formation, MTOC translocation and T cell signaling and activation. Time-lapse videomicroscopy and two-photon in vivo imaging of pDC-T cell interactions revealed that immature pDCs preferentially mediated transient interactions, whereas mature pDCs promoted more stable contacts. Our data indicate that under steady state conditions pDCs preferentially establish transient contacts with naive T cells, and show a very modest immunogenic capability, whereas upon maturation, pDCs are able to form long-lived contacts with T cells, and significantly enhance their capacity to activate these lymphocytes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Teshima, M. Kadowaki, and M. Koyama Response: Recipient plasmacytoid dendritic cells and graft-versus-host disease Blood, August 6, 2009; 114(6): 1280 - 1280. [Full Text] [PDF] M. Koyama, D. Hashimoto, K. Aoyama, K.-i. Matsuoka, K. Karube, H. Niiro, M. Harada, M. Tanimoto, K. Akashi, and T. Teshima Plasmacytoid dendritic cells prime alloreactive T cells to mediate graft-versus-host disease as antigen-presenting cells Blood, February 26, 2009; 113(9): 2088 - 2095. [Abstract] [Full Text] [PDF]

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