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Blood, 15 November 2008, Vol. 112, No. 10, pp. 4292-4297. Prepublished online as a Blood First Edition Paper on August 8, 2008; DOI 10.1182/blood-2008-02-139915. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-02-139915v1 112/10/4292    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ganz, T. Articles by Westerman, M. Search for Related Content PubMed PubMed Citation Articles by Ganz, T. Articles by Westerman, M. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 20, 2008 Accepted July 31, 2008 Immunoassay for human serum hepcidin Tomas Ganz*, Gordana Olbina, Domenico Girelli, Elizabeta Nemeth, and Mark Westerman Intrinsic LifeSciences, LLC, La Jolla, CA, United States Department of Clinical and Experimental Medicine, University of Verona, Verona, Italy Medicine and Pathology, David Geffen School of Medicine, University of California, Los Angeles, CA, United States * Corresponding author; email: tganz{at}mednet.ucla.edu. We developed and validated the first serum ELISA for hepcidin, the principal iron-regulatory hormone that has been very difficult to measure. In healthy volunteers, the 5-95% range of hepcidin concentrations was 29-254 ng/ml in men (n=65) and 17-286 ng/ml in women (n=49), with median concentrations 112 vs. 65, p<0.001. The lower limit of detection was 5 ng/ml. Serum hepcidin concentrations in 24 healthy subjects correlated well with their urinary hepcidin (r=0.82). Serum hepcidin appropriately correlated with serum ferritin (r=0.63) reflecting the regulation of both proteins by iron stores. Healthy volunteers showed a diurnal increase of serum hepcidin at noon and 8pm compared to 8am, and a transient rise of serum hepcidin in response to iron ingestion. Expected alterations in hepcidin levels were observed in a variety of clinical conditions associated with iron disturbances. Serum hepcidin concentrations were undetectable or low in patients with iron deficiency anemia (ferritin<10 ng/ml), iron-depleted HFE hemochromatosis, and juvenile hemochromatosis. Serum hepcidin concentrations were high in patients with inflammation (CRP>10 mg/dl), multiple myeloma, or chronic kidney disease. The new serum hepcidin ELISA yields accurate and reproducible measurements that appropriately reflect physiologic, pathologic and genetic influences, and is informative about the etiology of iron disorders. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: An immunoassay for human serum hepcidin at last: Ganz klar? Carlo Brugnara Blood 2008 112: 3922-3923. [Full Text] [PDF] This article has been cited by other articles: M. B Zimmermann, B. Troesch, R. Biebinger, I. Egli, C. Zeder, and R. F Hurrell Plasma hepcidin is a modest predictor of dietary iron bioavailability in humans, whereas oral iron loading, measured by stable-isotope appearance curves, increases plasma hepcidin Am. J. Clinical Nutrition, November 1, 2009; 90(5): 1280 - 1287. [Abstract] [Full Text] [PDF] D. H. Rushton, R. Dover, M. J. Norris, and J. J. H. Gilkes Is the recommended daily iron intake for women too low? 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Fargion HFE Mutations Modulate the Effect of Iron on Serum Hepcidin-25 in Chronic Hemodialysis Patients Clin. J. Am. Soc. Nephrol., August 1, 2009; 4(8): 1331 - 1337. [Abstract] [Full Text] [PDF] B. Chung, T. Chaston, J. Marks, S. K. Srai, and P. A. Sharp Hepcidin Decreases Iron Transporter Expression in Vivo in Mouse Duodenum and Spleen and in Vitro in THP-1 Macrophages and Intestinal Caco-2 Cells J. Nutr., August 1, 2009; 139(8): 1457 - 1462. [Abstract] [Full Text] [PDF] R. L. Sham, P. D. Phatak, E. Nemeth, and T. Ganz Hereditary hemochromatosis due to resistance to hepcidin: high hepcidin concentrations in a family with C326S ferroportin mutation Blood, July 9, 2009; 114(2): 493 - 494. [Full Text] [PDF] L. Ukarma, H. Johannes, U. Beyer, M. Zaug, B. Osterwalder, and A. Scherhag Hepcidin as a Predictor of Response to Epoetin Therapy in Anemic Cancer Patients Clin. Chem., July 1, 2009; 55(7): 1354 - 1360. [Abstract] [Full Text] [PDF] G. Peslova, J. Petrak, K. Kuzelova, I. Hrdy, P. 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[Abstract] [Full Text] [PDF] I. Theurl, E. Aigner, M. Theurl, M. Nairz, M. Seifert, A. Schroll, T. Sonnweber, L. Eberwein, D. R. Witcher, A. T. Murphy, et al. Regulation of iron homeostasis in anemia of chronic disease and iron deficiency anemia: diagnostic and therapeutic implications Blood, May 21, 2009; 113(21): 5277 - 5286. [Abstract] [Full Text] [PDF] M.-L. Island, A.-M. Jouanolle, A. Mosser, Y. Deugnier, V. David, P. Brissot, and O. Loreal A new mutation in the hepcidin promoter impairs its BMP response and contributes to a severe phenotype in HFE related hemochromatosis Haematologica, May 1, 2009; 94(5): 720 - 724. [Abstract] [Full Text] [PDF] H. Huang, M. Constante, A. Layoun, and M. M. Santos Contribution of STAT3 and SMAD4 pathways to the regulation of hepcidin by opposing stimuli Blood, April 9, 2009; 113(15): 3593 - 3599. [Abstract] [Full Text] [PDF] M. A Roe, R. Collings, J. R Dainty, D. W Swinkels, and S. J Fairweather-Tait Plasma hepcidin concentrations significantly predict interindividual variation in iron absorption in healthy men Am. J. Clinical Nutrition, April 1, 2009; 89(4): 1088 - 1091. [Abstract] [Full Text] [PDF] D. M Frazer and G. J Anderson Hepcidin compared with prohepcidin: an absorbing story Am. J. Clinical Nutrition, February 1, 2009; 89(2): 475 - 476. [Full Text] [PDF] K. J.H. Robson and D. J. Weatherall Malarial anemia and STAT6 Haematologica, February 1, 2009; 94(2): 157 - 159. [Full Text] [PDF] An Immunoassay for Hepcidin, the Master Regulator of Iron Metabolism Journal Watch Oncology and Hematology, December 9, 2008; 2008(1209): 2 - 2. [Full Text]

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