SEARCH:   Advanced

Blood, 1 October 2008, Vol. 112, No. 7, pp. 2969-2972. Prepublished online as a Blood First Edition Paper on July 17, 2008; DOI 10.1182/blood-2008-03-145011. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-03-145011v1 112/7/2969    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dobson, J. T. Articles by Berman, J. N Search for Related Content PubMed PubMed Citation Articles by Dobson, J. T. Articles by Berman, J. N Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 28, 2008 Accepted June 13, 2008 Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determination J. Tristan Dobson, Jake Seibert, Evelyn M Teh, Sahar Daas, Robert B Fraser, Barry H Paw, Tong-Jun Lin, and Jason N Berman* IWK Health Centre, Dalhousie University, Halifax, NS, Canada Department of Microbiology and Immunology, IWK Health Centre, Dalhousie University, Halifax, NS, Canada Department of Pathology, IWK Health Centre, Dalhousie University, Halifax, NS, Canada Division of Hematology, Department of Medicine, Brigham & Women's Hospital, Children's Hospital and Harvard Medical School, Boston, MA, United States Department of Pediatrics, IWK Health Centre, Dalhousie University, Halifax, NS, Canada * Corresponding author; email: jason.berman{at}iwk.nshealth.ca. Mast cells (MCs) play critical roles in allergy and inflammation, yet their development remains controversial due to limitations posed by traditional animal models. The zebrafish provides a highly efficient system for studying vertebrate hematopoiesis. We have identified zebrafish MCs in the gill and intestine, which resemble their mammalian counterparts both structurally and functionally. Carboxypeptidase A5 (cpa5), a MC specific enzyme, is expressed in zebrafish blood cells beginning at 24 hours post fertilization (hpf). At 28 hpf, co-localization is observed with pu.1, mpo, l-plastin and lysozyme C, but not fms or cepb, identifying these early MCs as a distinct myeloid population arising from a common granulocyte/monocyte progenitor. Morpholino "knockdown" studies demonstrate transcription factors gata-2 and pu.1, but not gata-1 or fog-1 as necessary for early MC development. These studies validate the zebrafish as an in vivo tool for studying MC ontogeny and function, with future capacity for modeling human MC diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. D. Amigo, G. E. Ackermann, J. J. Cope, M. Yu, J. D. Cooney, D. Ma, N. B. Langer, E. Shafizadeh, G. C. Shaw, W. Horsely, et al. The role and regulation of friend of GATA-1 (FOG-1) during blood development in the zebrafish Blood, November 19, 2009; 114(21): 4654 - 4663. [Abstract] [Full Text] [PDF] O. B. Dale, B. Torud, A. Kvellestad, H. S. Koppang, and E. O. Koppang From Chronic Feed-Induced Intestinal Inflammation to Adenocarcinoma with Metastases in Salmonid Fish Cancer Res., May 15, 2009; 69(10): 4355 - 4362. [Abstract] [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020