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Blood, 15 October 2008, Vol. 112, No. 8, pp. 3517-3525. Prepublished online as a Blood First Edition Paper on June 9, 2008; DOI 10.1182/blood-2008-03-145391. This Article Full Text (PDF) All Versions of this Article: blood-2008-03-145391v1 112/8/3517    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Friedman, T. M. Articles by Korngold, R. Search for Related Content PubMed PubMed Citation Articles by Friedman, T. M. Articles by Korngold, R. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 18, 2008 Accepted May 24, 2008 Overlap between in vitro donor anti-host and in vivo post-transplantation TCR V utilization: A new paradigm for designer allogeneic blood and marrow transplantation Thea M. Friedman*, Kira Goldgirsh, Stephanie A. Berger, Jenny Zilberberg, Joanne Filicko-O'Hara, Neal Flomenberg, Michele Donato, Scott D. Rowley, and Robert Korngold Cancer Center, Hackensack University Medical Center, Hackensack, New Jersey, United States Hematologic Malignancies, Blood and Marrow Transplant Program; Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United States Touro University College of Medicine, Hackensack, New Jersey, United States * Corresponding author; email: tfriedman{at}humed.com. Following allogeneic blood and marrow transplantation (BMT), mature donor T cells can enhance engraftment, counteract opportunistic infections, and mount graft-versus-tumor (GVT) responses, but at the risk of developing graft-versus-host disease (GVHD). With the aim of separating the beneficial effects of donor T cells from GVHD, one approach would be to selectively deplete subsets of alloreactive T cells in the hematopoietic cell inoculum. In this regard, TCR V repertoire analysis by CDR3-size spectratyping can be a powerful tool for the characterization of alloreactive T cell responses. We investigated the potential of this spectratype approach by comparing the donor T cell alloresponses generated in vitro against patient peripheral blood lymphocytes (PBL) with those detected in vivo post-transplantation. The results indicated that for most V families that exhibited alloreactive CDR3-size skewing, there was a robust overlap between the in vitro anti-patient and in vivo spectratype histograms. Thus, in vitro spectratype analysis may be useful for determining the alloreactive T cell response involved in GVHD development and, thereby, could serve to guide select V family-depletion for designer transplants to improve outcomes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Raising the spectra of T-cell profiling William J. Murphy Blood 2008 112: 3008-3009. [Full Text] [PDF] This article has been cited by other articles: H. Zheng, C. Matte-Martone, D. Jain, J. McNiff, and W. D. Shlomchik Central Memory CD8+ T Cells Induce Graft-versus-Host Disease and Mediate Graft-versus-Leukemia J. Immunol., May 15, 2009; 182(10): 5938 - 5948. [Abstract] [Full Text] [PDF]

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