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Blood, 1 November 2008, Vol. 112, No. 9, pp. 3713-3722. Prepublished online as a Blood First Edition Paper on August 12, 2008; DOI 10.1182/blood-2008-03-146290. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-03-146290v1 112/9/3713    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mouries, J. Articles by Leclerc, C. Search for Related Content PubMed PubMed Citation Articles by Mouries, J. Articles by Leclerc, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 21, 2008 Accepted July 4, 2008 Plasmacytoid dendritic cells efficiently cross-prime naive T cells in vivo after TLR activation Juliette Mouries, Gabriel Moron, Geraldine Schlecht, Nicolas Escriou, Gilles Dadaglio, and Claude Leclerc* Regulation Immunitaire et Vaccinologie, Institut Pasteur, Paris, France Genetique Moleculaire des Virus Respiratoires, Institut Pasteur, Paris, France INSERM, U 883, Paris, France * Corresponding author; email: cleclerc{at}pasteur.fr. Cross-presentation is a crucial mechanism in tumoral and microbial immunity because it allows internalized cell associated or exogenous antigens (Ags) to be delivered into the MHC-I pathway. This pathway is important for the development of CD8+ T-cell responses and for the induction of tolerance. In mice, cross-presentation is considered to be a unique property of CD8+ conventional dendritic cells (DCs). Here, we show that splenic plasmacytoid DCs (pDCs), efficiently capture exogenous Ags in vivo, but are not able to cross-present these Ags at steady state. However, in vitro and in vivo stimulation by Toll like receptor-7, or -9 or viruses licenses pDCs to cross-present soluble or particulate Ags by a TAP-dependent mechanism. Induction of cross-presentation confers to pDCs the ability to generate efficient effector CD8+ T-cell responses against exogenous Ags in vivo showing that pDCs may play a crucial role in induction of adaptive immune responses against pathogens that do not infect tissues of haemopoietic origin. This study provides the first evidence for an in vivo role of splenic pDCs in Ag cross presentation and T-cell cross-priming and suggests that pDCs may constitute an attractive target to boost the efficacy of vaccines based on CTL induction. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Flores, D. D. Desai, M. Downie, B. Liang, M. P. Reilly, S. E. McKenzie, and R. Clynes Dominant Expression of the Inhibitory Fc{gamma}RIIB Prevents Antigen Presentation by Murine Plasmacytoid Dendritic Cells J. Immunol., December 1, 2009; 183(11): 7129 - 7139. [Abstract] [Full Text] [PDF] D. A. Carlow, M. R. Gold, and H. J. Ziltener Lymphocytes in the Peritoneum Home to the Omentum and Are Activated by Resident Dendritic Cells J. Immunol., July 15, 2009; 183(2): 1155 - 1165. [Abstract] [Full Text] [PDF]

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