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Blood, 1 December 2008, Vol. 112, No. 12, pp. 4523-4531. Prepublished online as a Blood First Edition Paper on September 4, 2008; DOI 10.1182/blood-2008-03-148502. This Article Full Text (PDF) All Versions of this Article: blood-2008-03-148502v1 112/12/4523    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tucker, K. L Articles by Gibbins, J. M Search for Related Content PubMed PubMed Citation Articles by Tucker, K. L Articles by Gibbins, J. M Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 28, 2008 Accepted August 11, 2008 A dual role for integrin linked kinase in platelets: regulating integrin function and -granule secretion Katherine L Tucker*, Tanya Sage, Joanne M Stevens, Peter A Jordan, Sarah Jones, Natasha E Barrett, Rene St-Arnaud, Jonathan Frampton, Shoukat Dedhar, and Jonathan M Gibbins School of Biological Sciences, The University of Reading, Reading, United Kingdom Department of Surgery and Department of Human Genetics, McGill University, Montreal, Quebec, Canada Division of Immunity and Infection, Birmingham University Medical School, Birmingham, United Kingdom Cancer Genetics and Developmental Biology, The BC Cancer Research Centre, Vancouver, British Columbia, Canada * Corresponding author; email: k.l.tucker{at}rdg.ac.uk. Integrin linked kinase (ILK) has been implicated in the regulation of a range of fundamental biological processes such as cell survival, growth, differentiation and adhesion. In platelets ILK associates with 1 and 3 containing integrins, which are of paramount importance for the function of platelets. Upon stimulation of platelets this association with the integrins is increased and ILK kinase activity is up-regulated, suggesting that ILK may be important for the co-ordination of platelet responses. In this study a conditional knockout mouse model was developed to examine the role of ILK in platelets. The ILK-deficient mice showed an increased bleeding time and volume, and despite normal ultra structure the function of ILK-deficient platelets was decreased significantly. This included reduced aggregation, fibrinogen binding and thrombus formation under arterial flow conditions. Furthermore, although early collagen stimulated signalling such as PLC2 phosphorylation and calcium mobilisation were unaffected in ILK-deficient platelets, a selective defect in -granule, but not dense-granule, secretion was observed. These results indicate that as well as involvement in the control of integrin affinity, ILK is required for -granule secretion and therefore may play a central role in the regulation of platelet function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Honda, H. Shirotani-Ikejima, S. Tadokoro, Y. Maeda, T. Kinoshita, Y. Tomiyama, and T. Miyata Integrin-linked kinase associated with integrin activation Blood, May 21, 2009; 113(21): 5304 - 5313. [Abstract] [Full Text] [PDF] D. S. Harburger and D. A. Calderwood Integrin signalling at a glance J. Cell Sci., January 15, 2009; 122(2): 159 - 163. [Full Text] [PDF]

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