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Blood, 15 September 2008, Vol. 112, No. 6, pp. 2287-2296. Prepublished online as a Blood First Edition Paper on July 1, 2008; DOI 10.1182/blood-2008-04-150268. This Article Full Text (PDF) Supplemental Figures and Videos All Versions of this Article: blood-2008-04-150268v1 112/6/2287    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Costa, R. M. B. Articles by Amaya, E. Search for Related Content PubMed PubMed Citation Articles by Costa, R. M. B. Articles by Amaya, E. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 11, 2008 Accepted June 23, 2008 spib is required for primitive myeloid development in Xenopus Ricardo M. B. Costa, Ximena Soto, Yaoyao Chen, Aaron M Zorn, and Enrique Amaya* The Healing Foundation Centre/ Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom Division of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH, United States * Corresponding author; email: enrique.amaya{at}manchester.ac.uk. Vertebrate blood formation occurs in two spatially and temporally distinct waves, so called primitive and definitive hematopoiesis. While definitive hematopoiesis has been extensively studied, the development of primitive myeloid blood has received far less attention. In Xenopus primitive myeloid cells originate in the anterior ventral blood islands, the equivalent of the mammalian yolk sac, and migrate out to colonize the embryo. Using fluorescence time-lapse video microscopy, we recorded the migratory behaviour of primitive myeloid cells from their birth. We show that these cells are the first blood cells to differentiate in the embryo and that they are efficiently recruited to embryonic wounds, well before the establishment of a functional vasculature. Furthermore, we isolated spib, an ETS transcription factor, specifically expressed in primitive myeloid precursors. Using spib antisense morpholino knock down experiments, we show that spib is required for myeloid specification, and, in its absence, primitive myeloid cells retain hemangioblast-like characteristics and fail to migrate. Thus, we conclude that spib sits at the top of the known genetic hierarchy that leads to the specification of primitive myeloid cells in amphibians. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Chen, R. M. B. Costa, N. R. Love, X. Soto, M. Roth, R. Paredes, and E. Amaya C/EBP{alpha} initiates primitive myelopoiesis in pluripotent embryonic cells Blood, July 2, 2009; 114(1): 40 - 48. [Abstract] [Full Text] [PDF]

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