SEARCH:   Advanced

Blood, 15 November 2008, Vol. 112, No. 10, pp. 4227-4234. Prepublished online as a Blood First Edition Paper on August 29, 2008; DOI 10.1182/blood-2008-04-151498. This Article Full Text (PDF) All Versions of this Article: blood-2008-04-151498v1 112/10/4227    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Orzechowska, B. U Articles by Wong, S. W Search for Related Content PubMed PubMed Citation Articles by Orzechowska, B. U Articles by Wong, S. W Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 17, 2008 Accepted July 7, 2008 Rhesus macaque rhadinovirus-associated non-Hodgkin's lymphoma: animal model for KSHV associated malignancies Beata U Orzechowska, Michael F Powers, Jerald Sprague, He Li, Bonnie Yen, Robert P Searles, Michael K Axthelm, and Scott W Wong* Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, United States Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, United States Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR, United States * Corresponding author; email: wongs{at}ohsu.edu. Rhesus macaque rhadinovirus (RRV) is closely related to Kaposi's sarcoma-associated herpesvirus (KSHV) and is associated with the development of B cell hyperplasia and persistent lymphadenopathy resembling multi-centric Castleman's disease (MCD) in rhesus macaques (RM) co-infected with simian immunodeficiency virus (SIV). Here, we investigated whether RM experimentally infected with SIV and RRV can develop other disease manifestations that are observed in HIV and KSHV-infected individuals. As reported earlier, experimental inoculation of SIV-infected RM with RRV results in persistent RRV infection, whereas immunocompetent animals infected with RRV exhibit viremia two weeks post-infection, followed by a period of no virus detection until they are subsequently made immunodeficient by SIV-infection. A subset of animals developed abnormal cellular proliferations characterized as extra-nodal lymphoma and a proliferative mesenchymal lesion. In situ hybridization and immunohistochemistry analysis indicate RRV is present in both malignancies and DNA microarray analysis detected viral interleukin-6 (vIL-6) and viral FLIP transcripts. Subsequent reverse-transcriptase PCR analysis confirmed vIL-6 and vFLIP expression, and that of RRV ORFs 72 and 73, all homologues of the KSHV ORFs shown to be expressed in primary effusion lymphoma. These data support the utility of the RRV/SIV-infected rhesus macaque as an excellent animal model to investigate KSHV-like pathogenesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. W. Wen, D. P. Dittmer, and B. Damania Disruption of LANA in Rhesus Rhadinovirus Generates a Highly Lytic Recombinant Virus J. Virol., October 1, 2009; 83(19): 9786 - 9802. [Abstract] [Full Text] [PDF] M. Okroj, L. Mark, A. Stokowska, S. W. Wong, N. Rose, D. J. Blackbourn, B. O. Villoutreix, O. B. Spiller, and A. M. Blom Characterization of the Complement Inhibitory Function of Rhesus Rhadinovirus Complement Control Protein (RCP) J. Biol. Chem., January 2, 2009; 284(1): 505 - 514. [Abstract] [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020