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Blood, 15 October 2008, Vol. 112, No. 8, pp. 3484-3487. Prepublished online as a Blood First Edition Paper on August 12, 2008; DOI 10.1182/blood-2008-05-157511. This Article Full Text (PDF) All Versions of this Article: blood-2008-05-157511v1 112/8/3484    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Woolfrey, A. E. Articles by Storb, R. Search for Related Content PubMed PubMed Citation Articles by Woolfrey, A. E. Articles by Storb, R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 15, 2008 Accepted July 25, 2008 Generation of HIV-1 specific CD8+ cell responses following allogeneic hematopoietic cell transplant Ann E. Woolfrey*, Uma Malhotra, Robert D Harrington, John McNevin, Thomas J Manley, Stanley R. Riddell, Robert W. Coombs, Frederick R. Appelbaum, Larry Corey, and Rainer Storb Fred Hutchinson Cancer Research Center, Seattle, WA, United States Infectious Diseases, Virginia Mason Medical Center, Seattle, WA, United States Infectious Disease, University of Washington, Seattle, WA, United States * Corresponding author; email: awoolfre{at}fhcrc.org. This study tested whether donor-derived HIV-specific immune responses could be detected when viral replication was completely suppressed by the continuous administration of highly active anti-retroviral therapy (HAART). A regimen of fludarabine and 200 cGy total body irradiation was followed by infusion of allogeneic donor peripheral blood cells and post-transplant cyclosporine and mycophenolate mofetil. Viral load, lymphocyte counts, and HIV-1-specific CD8+ cell immune responses were compared before and after HCT. Uninterrupted administration of HAART was feasible during nonmyeloablative conditioning and following HCT. The HIV RNA remained undetectable and no HIV-associated infections were observed. CD8+ T-cell responses targeting multiple epitopes were detected prior to HCT. Following HCT a different pattern of donor-derived HIV-specific CTL responses emerged by day +80, presumably primed in vivo. We conclude that allogeneic HCT offers the unique ability to characterize de novo HIV-1-specific immune responses. This clinical trial was registered at ClinicalTrials.gov Identifier: NCT00112593. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Hamadani and S. M. Devine Reduced-intensity conditioning allogeneic stem cell transplantation in HIV patients with hematologic malignancies: yes, we can Blood, September 17, 2009; 114(12): 2564 - 2566. [Full Text] [PDF]

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