Submitted May 21, 2008
Accepted September 24, 2008
Integrin 
v
3 on human endothelial cells binds von Willebrand factor strings under fluid shear stress
Jing Huang, Robyn Roth, John E Heuser, and J Evan Sadler*
Department of Medicine, Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO, United States
Department of Cell Biology, Washington University School of Medicine, St. Louis, MO, United States
* Corresponding author; email: esadler{at}im.wustl.edu.
Acutely secreted von Willebrand factor (VWF) multimers adhere to endothelial cells, support platelet adhesion, and may induce microvascular thrombosis. Immunofluorescence microscopy of live human umbilical vein endothelial cells showed that VWF multimers rapidly formed strings several hundred micrometers long on the cell surface after stimulation with histamine. Unexpectedly, only a subset of VWF strings supported platelet binding, which depended on platelet glycoprotein Ib. Electron microscopy showed that VWF strings often consisted of bundles and networks of VWF multimers, and each string was tethered to the cell surface by a limited number of sites. Several approaches implicated P-selectin and integrin 
v
3 in anchoring VWF strings. An RGDS peptide or a function-blocking antibody to integrin v3 reduced the number of VWF strings formed. Also, integrin v decorated the VWF strings by immunofluorescence microscopy. Furthermore, lentiviral transduction of shRNA against the v subunit reduced the expression of cell surface integrin v3 and impaired the ability of endothelial cells to retain VWF strings. Soluble P-selectin reduced the number of platelet-decorated VWF strings in the absence of Ca2+ and Mg2+, but had no effect in the presence of these cations. These results indicate that VWF strings bind specifically to integrin v3 on human endothelial cells.
