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Blood, 5 February 2009, Vol. 113, No. 6, pp. 1294-1303. Prepublished online as a Blood First Edition Paper on October 15, 2008; DOI 10.1182/blood-2008-05-158865. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2008-05-158865v1 113/6/1294    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Grubor, V. Articles by Esposito, D. Search for Related Content PubMed PubMed Citation Articles by Grubor, V. Articles by Esposito, D. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 23, 2008 Accepted August 23, 2008 Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA) Vladimir Grubor, Alex Krasnitz, Jennifer E. Troge, Jennifer L. Meth, B. Lakshmi, Jude T. Kendall, Boris Yamrom, Garrick Alex, Deepa Pai, Nicholas Navin, Lisa A. Hufnagel, Yoon-Ha Lee, Kerry Cook, Steven L. Allen, Kanti R. Rai, Rajendra N. Damle, Carlo Calissano, Nicholas Chiorazzi, Michael Wigler, and Diane Esposito* Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, United States North Shore University Hospital, North Shore LIJ Health System, Manhasset, NY, United States Long Island Jewish Medical Center, North Shore LIJ Health System, New Hyde Park, NY, United States The Feinstein Institute for Medical Research, North Shore LIJ Health System, Manhasset, NY, United States * Corresponding author; email: esposito{at}cshl.edu. We examined copy number changes in the genomes of B cells from 58 patients with chronic lymphocytic leukemia (CLL) using representational oligonucleotide microarray analysis (ROMA), a form of comparative genomic hybridization (CGH), at a resolution exceeding previously published studies. We observed at least one genomic lesion in each CLL sample and considerable variation in the number of abnormalities from case to case. Virtually all abnormalities previously reported were also observed here, most of which were indeed highly recurrent. We observed the boundaries of known events with greater clarity and identified previously undescribed lesions, some of which were recurrent. We profiled the genomes of CLL cells separated by the surface marker CD38, and found evidence of distinct subclones of CLL within the same patient. We discuss the potential applications of high resolution CGH analysis in a clinical setting. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: ROMA illuminates CLL genomic lesions Federico Caligaris-Cappio Blood 2009 113: 1209-1210. [Full Text] [PDF] This article has been cited by other articles: C. Calissano, R. N. Damle, G. Hayes, E. J. Murphy, M. K. Hellerstein, C. Moreno, C. Sison, M. S. Kaufman, J. E. Kolitz, S. L. Allen, et al. In vivo intraclonal and interclonal kinetic heterogeneity in B-cell chronic lymphocytic leukemia Blood, November 26, 2009; 114(23): 4832 - 4842. [Abstract] [Full Text] [PDF]

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