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Blood, 2 April 2009, Vol. 113, No. 14, pp. 3363-3370. Prepublished online as a Blood First Edition Paper on February 4, 2009; DOI 10.1182/blood-2008-05-160325. This Article Full Text (PDF) Supplemental Methods, Tables, and Figure All Versions of this Article: blood-2008-05-160325v1 113/14/3363    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yamamoto, M. L. Articles by Conboy, J. G. Search for Related Content PubMed PubMed Citation Articles by Yamamoto, M. L. Articles by Conboy, J. G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 30, 2008 Accepted December 2, 2008 Alternative pre-mRNA splicing switches modulate gene expression in late erythropoiesis Miki L. Yamamoto, Tyson A. Clark, Sherry L. Gee, Jeong-Ah Kang, Anthony C. Schweitzer, Amittha Wickrema, and John G. Conboy* Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States Affymetrix, Inc., Santa Clara, CA, United States Department of Medicine, University of Chicago, Chicago, IL, United States * Corresponding author; email: jgconboy{at}lbl.gov. Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression, but cannot detect qualitative changes in transcript structure, mediated by RNA processing, that alter structure and function of encoded proteins. We analyzed stage-specific changes in the late erythroid transcriptome via use of high resolution microarrays that detect altered expression of individual exons. Ten differentiation-associated changes in erythroblast splicing patterns were identified, including the previously known activation of protein 4.1R exon 16 splicing. Six new alternative splicing switches involving enhanced inclusion of internal cassette exons were discovered, as well as three changes in use of alternative first exons. All of these erythroid stage-specific splicing events represent activated inclusion of authentic annotated exons, suggesting they represent an active regulatory process rather than a general loss of splicing fidelity. The observation that three of the regulated transcripts encode RNA binding proteins (SNRP70, HNRPLL, MBNL2) may indicate significant changes in the RNA processing machinery of late erythroblasts. Together these results support the existence of a regulated alternative pre-mRNA splicing program that is critical for late erythroid differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. A. Steiner, Y. Maksimova, V. Schulz, C. Wong, D. Raha, M. C. Mahajan, S. M. Weissman, and P. G. Gallagher Chromatin Architecture and Transcription Factor Binding Regulate Expression of Erythrocyte Membrane Protein Genes Mol. Cell. Biol., October 15, 2009; 29(20): 5399 - 5412. [Abstract] [Full Text] [PDF] L. Lin, S. Liu, H. Brockway, J. Seok, P. Jiang, W. H. Wong, and Y. Xing Using high-density exon arrays to profile gene expression in closely related species Nucleic Acids Res., July 1, 2009; 37(12): e90 - e90. [Abstract] [Full Text] [PDF]

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