Submitted July 11, 2008
Accepted October 11, 2008
Gene expression profiling of peripheral T-cell lymphoma including 
T-cell lymphoma
Kana Miyazaki, Motoko Yamaguchi*, Hiroshi Imai, Tohru Kobayashi, Satoshi Tamaru, Kazuhiro Nishii, Masao Yuda, Hiroshi Shiku, and Naoyuki Katayama
Department of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu, Japan
Department of Pathologic Oncology, Mie University Graduate School of Medicine, Tsu, Japan
Department of Medical Zoology, Mie University Graduate School of Medicine, Tsu, Japan
Department of Cancer Vaccine and Immuno-Gene Therapy, Mie University Graduate School of Medicine, Tsu, Japan
* Corresponding author; email: waniwani{at}clin.medic.mie-u.ac.jp.
The gene expression profile of peripheral 
T-cell lymphoma (TCL) has not been investigated. Using oligonucleotide microarrays, we analyzed total RNA from 7 patients with TCL (4 hepatosplenic, 1 cutaneous, 1 intestinal, and 1 thyroidal) and 27 patients with 
T-cell lymphoma (TCL) (11 peripheral T-cell lymphoma-unspecified, 15 angioimmunoblastic T-cell lymphoma, and 1 hepatosplenic). Unsupervised microarray analyses classified all hepatosplenic TCLs into a single cluster, whereas other TCLs were scattered within the TCL distribution. We identified a T-cell receptor signature gene set which accurately classified TCL and TCL. A classifier based on gene expression under supervised analysis correctly identified TCL. One case of hepatosplenic TCL was placed in the TCL grouping. TCL signature genes included genes encoding killer cell immunoglobulin-like receptors and killer cell lectin-like receptors. Our results indicate that hepatosplenic TCL is a distinct form of peripheral T-cell lymphoma and suggest that nonhepatosplenic TCLs are heterogeneous in gene expression.
