SEARCH:   Advanced

Blood, 8 January 2009, Vol. 113, No. 2, pp. 273-278. Prepublished online as a Blood First Edition Paper on October 2, 2008; DOI 10.1182/blood-2008-07-167098. This Article Full Text (PDF) Supplemental Methods, Tables, and Figures All Versions of this Article: blood-2008-07-167098v1 113/2/273    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Paczesny, S. Articles by Ferrara, J. L. M. Search for Related Content PubMed PubMed Citation Articles by Paczesny, S. Articles by Ferrara, J. L. M. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 8, 2008 Accepted September 10, 2008 A biomarker panel for acute graft versus host disease Sophie Paczesny*, Oleg I Krijanovski, Thomas M Braun, Sung Won Choi, Shawn G. Clouthier, Rork Kuick, David E. Misek, Kenneth R Cooke, Carrie L Kitko, Angela Weyand, Daniel Bickley, Dawn Jones, Joel Whitfield, Pavan Reddy, John E Levine, Samir M. Hanash, and James L. M. Ferrara Department of Pediatrics, University of Michigan, Ann Arbor, MI, United States Department of Internal Medicine, University of Michigan, Ann Arbor, MI, United States Department of Biostatistics, University of Michigan, Ann Arbor, MI, United States Comprehensive Cancer Center, Biostatistics Core, University of Michigan, Ann Arbor, MI, United States Department of Surgery, University of Michigan, Ann Arbor, MI, United States Department of Pediatrics, Case Western Reserve University, Cleveland, OH, United States Fred Hutchinson Cancer Research Center, Seattle, WA, United States * Corresponding author; email: sophiep{at}umich.edu. No validated biomarkers exist for acute graft versus host disease (GVHD). We screened plasma with antibody microarrays for 120 proteins in a discovery set of 42 transplant patients that revealed eight potential biomarkers for diagnostic of GVHD. We then measured by ELISA the levels of these biomarkers in samples from 424 transplant patients randomly divided into training (n = 282) and validation (n = 142) sets. Logistic regression analysis of these eight proteins determined a composite biomarker panel of four proteins (interleukin-2-receptor-alpha, tumor-necrosis-factor-receptor-1, interleukin-8, and hepatocyte growth factor) that optimally discriminated patients with and without GVHD. The area under the receiver operating characteristic curve distinguishing these two groups in the training set was 0.91 [95% confidence interval, 0.87 - 0.94] and 0.86 [95% confidence interval, 0.79 - 0.92] in the validation set. In patients with GVHD, Cox regression analysis revealed that the biomarker panel predicted survival independently of GVHD severity. A panel of four biomarkers can confirm the diagnosis of GVHD in patients at onset of clinical symptoms of GVHD and provide prognostic information independent of GVHD severity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Graft-versus-host disease: proteomics comes of age Gerard Socié Blood 2009 113: 271-272. [Full Text] [PDF] This article has been cited by other articles: G. Socie and B. R. Blazar Acute graft-versus-host disease: from the bench to the bedside Blood, November 12, 2009; 114(20): 4327 - 4336. [Abstract] [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020