SEARCH:   Advanced

Blood, 11 June 2009, Vol. 113, No. 24, pp. 6112-6119. Prepublished online as a Blood First Edition Paper on April 14, 2009; DOI 10.1182/blood-2008-07-170803. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: blood-2008-07-170803v1 113/24/6112    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nascimbeni, M. Articles by Hosmalin, A. Search for Related Content PubMed PubMed Citation Articles by Nascimbeni, M. Articles by Hosmalin, A. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 28, 2008 Accepted March 25, 2009 Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients, but barely participate in interferon alpha expression Michelina Nascimbeni, Leila Perie, Laurent Chorro, Seckou Diocou, Louis Kreitmann, Stephanie Louis, Laurent Garderet, Bettina Fabiani, Anne Berger, Jurgen Schmitz, Jean-Pierre Marie, Thierry Jo Molina, Jerome Pacanowski, Jean-Paul Viard, Eric Oksenhendler, Stephanie Beq, Orly Abehsira-Amar, Remi Cheynier, and Anne Hosmalin* Departement d'Immunologie, Institut Cochin, Paris, France Institut National de la Sante et de la Recherche Medicale, Unite 567, Paris, France Centre National de la Recherche Scientifique, UMR 8104, Paris, France Universite Paris Descartes, Paris, France Hematologie, Anatomie pathologique, Hopital Saint-Antoine, Paris, France Chirurgie Generale et Digestive, Hopital Europeen Georges Pompidou, Paris, France Miltenyi Biotech GmbH, Bergisch Gladbach, Germany AP-HP, Hotel-Dieu, Departement d'hematologie et d'oncologie medicale, Paris, France AP-HP, Hotel-Dieu, Service d'Anatomie et Cytologie Pathologiques, Paris, France Service des Maladies Infectieuses, Hopital Necker, Paris, France Service d'Immunologie Clinique, Hopital Saint-Louis, Paris, France Institut Pasteur, Departement de Virologie, Paris, France * Corresponding author; email: anne.hosmalin{at}inserm.fr. We characterized the localization, phenotype and some functions of plasmacytoid dendritic cells (pDC) in the human spleen. pDC were localized in the marginal zone and the peri-arteriolar region. Some were also found in the red pulp. pDC were immature by phenotypic labeling, consistently with their capacity to internalize Dextran in a functional assay. In spleens from human immunodeficiency virus (HIV)-infected patients with thrombocytopenic purpura, these characteristics were unaffected. However, an accumulation of pDC, but not mDC, was observed in some HIV+ patients, correlating with high proviral loads. Moreover, while undetectable in most HIV- patients, IFN- production was evidenced in situ and by flow cytometry in most HIV+ patients. IFN- was located in the marginal zone. Surprisingly, IFN- colocalized only with few pDC, but rather with other cells, including T and B lymphocytes, mDC and macrophages. Therefore pDC accumulated in spleens from HIV+ patients with high proviral loads, but they did not seem to be the main IFN- producers. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020