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Blood, 22 January 2009, Vol. 113, No. 4, pp. 807-815. Prepublished online as a Blood First Edition Paper on October 16, 2008; DOI 10.1182/blood-2008-08-173682. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-08-173682v1 113/4/807    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Serwold, T. Articles by Weissman, I. L. Search for Related Content PubMed PubMed Citation Articles by Serwold, T. Articles by Weissman, I. L. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 13, 2008 Accepted September 25, 2008 Reductive isolation from bone marrow and blood implicates common lymphoid progenitors as the major source of thymopoiesis Thomas Serwold, Lauren I Richie Ehrlich*, and Irving L. Weissman Stanford Institute of Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, United States Stanford Ludwig Center for Stem Cell Research, Stanford University, Stanford, CA, United States * Corresponding author; email: lauren.ehrlich{at}stanford.edu. Ongoing thymopoiesis requires continual seeding from progenitors that reside within the bone marrow (BM), but the identity of the most proximate pre-thymocytes has remained controversial. Here we take a comprehensive approach to prospectively identify the major source of thymocyte progenitors that reside within the BM and blood, and find that all thymocyte progenitor activity resides within a rare Flk2+CD27+ population. The BM Flk2+CD27+ subset is predominantly composed of common lymphoid progenitors (CLP) and multipotent progenitors (MPP). Of these two populations, only CLP reconstitute thymopoiesis rapidly after intravenous injection. In contrast, MPP-derived cells reconstitute the thymus with delayed kinetics only after they have reseeded the BM, self-renewed and generated CLP. These results identify CLP as the major source of thymocyte progenitors within the BM. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: CLPs find their inner T-cell potential Kevin D. Bunting Blood 2009 113: 766-767. [Full Text] [PDF] This article has been cited by other articles: E. Fiorini, E. Merck, A. Wilson, I. Ferrero, W. Jiang, U. Koch, F. Auderset, E. Laurenti, F. Tacchini-Cottier, M. Pierres, et al. Dynamic Regulation of Notch 1 and Notch 2 Surface Expression during T Cell Development and Activation Revealed by Novel Monoclonal Antibodies J. Immunol., December 1, 2009; 183(11): 7212 - 7222. [Abstract] [Full Text] [PDF] D. Metcalf, A. P. Ng, S. J. L. Loughran, B. Phipson, G. K. Smyth, L. Di Rago, and S. Mifsud Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profiles PNAS, November 10, 2009; 106(45): 19102 - 19107. [Abstract] [Full Text] [PDF] A. N. Vallejo, J. J. Michel, L. K. Bale, B. H. Lemster, L. Borghesi, and C. A. Conover Resistance to age-dependent thymic atrophy in long-lived mice that are deficient in pregnancy-associated plasma protein A PNAS, July 7, 2009; 106(27): 11252 - 11257. [Abstract] [Full Text] [PDF]

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