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 Blood, 26 February 2009, Vol. 113, No. 9, pp. 1977-1981.
Prepublished online as a Blood First Edition Paper on December 8, 2008; DOI 10.1182/blood-2008-08-174094.

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Submitted August 14, 2008
Accepted November 26, 2008

Activated T-cells recruit exosomes secreted by dendritic cells via LFA-1

Esther N.M. Nolte-'t Hoen, Sonja I. Buschow, Stephen M. Anderton, Willem Stoorvogel, and Marca H.M. Wauben*

Department of Biochemistry & Cell Biology, Utrecht University, Faculty of Veterinary Medicine, Utrecht, Netherlands
Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom

* Corresponding author; email: m.h.m.wauben{at}uu.nl.

Dendritic cells (DC) are known to secrete exosomes that transfer membrane proteins, like MHC-II, to other DC. Intercellular transfer of membrane proteins is also observed during cognate interactions between DC and CD4+ T-cells. The acquired proteins are functional and play a role in regulation of immune responses. How membrane protein transfer is achieved and regulated is unclear. Here we show that T-cells can recruit MHC-II-containing DC-exosomes secreted in the extracellular milieu during cognate DC-T-cell interactions. Recruitment of these exosomes required T-cell activation and was dependent on LFA-1 rather than on TCR specificity. In fact, inducing a high affinity state of LFA-1 on resting T-cells was sufficient to provoke exosome-binding. These results imply that DC-exosomes secreted in the extracellular milieu during cognate T-cell-DC interactions are targeted to T cells activated in that micro-environment.


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