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Blood, 18 June 2009, Vol. 113, No. 25, pp. 6477-6484. Prepublished online as a Blood First Edition Paper on March 3, 2009; DOI 10.1182/blood-2008-09-176594. This Article Full Text (PDF) Supplemental Table All Versions of this Article: blood-2008-09-176594v1 113/25/6477    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Clave, E. Articles by Toubert, A. Search for Related Content PubMed PubMed Citation Articles by Clave, E. Articles by Toubert, A. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 2, 2008 Accepted February 14, 2009 Acute graft versus host disease transiently impairs thymic output in young patients after allogeneic hematopoietic stem cell transplantation Emmanuel Clave*, Marc Busson, Corinne Douay, Regis Peffault de Latour, Jeannig Berrou, Claire Rabian, Maryvonnick Carmagnat, Vanderson Rocha, Dominique Charron, Gerard Socie, and Antoine Toubert Laboratoire d'Immunologie et d'Histocompatibilite AP-HP, INSERM UMRS-940, Institut Universitaire d'Hematologie, Universite Paris-Diderot, Paris, France Service d'Hematologie-Greffe de Moelle, Hopital Saint-Louis, AP-HP, Paris, France INSERM U728, Institut Universitaire d'Hematologie, Paris, France * Corresponding author; email: emmanuel.clave{at}univ-paris-diderot.fr. Long term T-cell reconstitution after Hematopoietic Stem cell Transplantation (HSCT) is dependent on patient thymic function and affected by Graft versus Host Disease (GvHD). To assess the impact of acute GvHD (aGvHD) on thymic function, we followed a cohort of 93 patients who received HSCT from an HLA-identical sibling, mainly for hematological malignancies. Thymic output was measured by "signal-joint T-cell Receptor Excision Circles" (sjTREC) real-time PCR. Absolute sjTREC number was lower at 6 months in patients with aGvHD (p=.014), associated with lower absolute counts of naïve CD4 T-cells at 6 and 12 months (p=.04 and .02), and persistent abnormalities in T-cell repertoire diversity. Age and aGvHD affected thymic function independently in multivariate analysis. In patients less than 25 years of age, thymic function recovered almost totally at one year. As a marker of thymocyte proliferation, we quantified the TREC generated during the TCR -chain recombination, in a group of 20 age-matched patients. Mean TREC level was reduced at 6 months in patients with aGvHD, indicating an impact on early thymic differentiation rather than on intrathymic proliferation. These data show that aGvHD or its treatment, has a transient impact on thymic function in younger patients in the first months after HSCT. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Is a little GVHD a good thing? Terry J. Fry Blood 2009 113: 6274-6275. [Abstract] [Full Text] [PDF] This article has been cited by other articles: T. J. Fry Is a little GVHD a good thing? Blood, June 18, 2009; 113(25): 6274 - 6275. [Abstract] [Full Text] [PDF]

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