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Blood, 30 April 2009, Vol. 113, No. 18, pp. 4250-4261. Prepublished online as a Blood First Edition Paper on January 23, 2009; DOI 10.1182/blood-2008-09-177741. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2008-09-177741v1 113/18/4250    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gaufin, T. Articles by Apetrei, C. Search for Related Content PubMed PubMed Citation Articles by Gaufin, T. Articles by Apetrei, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 8, 2008 Accepted January 7, 2009 Limited ability of humoral immune responses in control of viremia during infection with SIVsmmD215 strain Thaidra Gaufin, Rajeev Gautam, Melissa Kasheta, Ruy Ribeiro, Erin Ribka, Mary Barnes, Melissa Pattison, Coty Tatum, Jeanne MacFarland, David Montefiori, Amitinder Kaur, Ivona Pandrea, and Cristian Apetrei* Division of Microbiology, Tulane National Primate Research Center, Covington, LA, United States Division of Immunology, New England Primate Research Center, Southborough, MA, United States Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM, United States Division of Veterninary Medicine, Tulane National Primate Research Center, Covington, LA, United States Department of Surgery, Duke University, Durham, NC, United States Department of Pathology, School of Medicine, Tulane University, New Orleans, LA, United States Department of Tropical Medicine, School of Public Health, Tulane University, New Orleans, LA, United States * Corresponding author; email: capetrei{at}tulane.edu. We investigated the impact of rhesus macaque (RM) B cell depletion prior to inoculation with the isolate SIVsmmD215. Seven RMs were treated every three weeks with 50 mg/kg of an anti-CD20 antibody (Rituxan) starting seven days prior to inoculation for two months (n=4) and five months (n=3), respectively. Four control animals received no antibody. Three animals were completely depleted of CD20+ B cells, but four were only partially depleted of CD20 cells in the LNs and intestine. The decrease in antibody production was consistent with the efficacy of tissue CD20 depletion. Seroconversion and neutralizing antibody production was significantly delayed in animals showing complete tissue CD20 depletion and remained at low titers in all CD20-depleted RMs. Surprisingly, there was no significant difference in acute or chronic viral loads between CD20-depleted and control animal groups. There was a tendency for lower viral set-points in CD20-depleted animals. At 6 weeks post-inoculation, cellular immune responses were significantly stronger in CD20-depleted animals than in controls. There was no significant difference in survival between CD20-depleted and control animals. Our data suggest that a deficiency of Ab responses did not markedly affect viral replication or disease progression and that they may be compensated by more robust cellular responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Gaufin, M. Pattison, R. Gautam, C. Stoulig, J. Dufour, J. MacFarland, D. Mandell, C. Tatum, M. H. Marx, R. M. Ribeiro, et al. Effect of B-Cell Depletion on Viral Replication and Clinical Outcome of Simian Immunodeficiency Virus Infection in a Natural Host J. Virol., October 15, 2009; 83(20): 10347 - 10357. [Abstract] [Full Text] [PDF]

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