SEARCH:   Advanced

Blood, 23 April 2009, Vol. 113, No. 17, pp. 4086-4093. Prepublished online as a Blood First Edition Paper on November 20, 2008; DOI 10.1182/blood-2008-09-181073. This Article Full Text (PDF) All Versions of this Article: blood-2008-09-181073v1 113/17/4086    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, W. Articles by Karpatkin, S. Search for Related Content PubMed PubMed Citation Articles by Zhang, W. Articles by Karpatkin, S. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 25, 2008 Accepted November 12, 2008 Role of molecular mimickry of hepatitis C-virus (HCV) protein with platelet GPIIIa in hepatitis C-related immunologic thrombocytopenia Wei Zhang, Michael A Nardi, Zongdong Li, William Borkowsky, and Simon Karpatkin* Department of Medicine, New York University School of Medicine, New York, NY, United States Department of Pediatrics, New York University School of Medicine, New York, NY, United States * Corresponding author; email: simon.karpatkin{at}med.nyu.edu. HIV-1-ITP patients have a unique Ab against platelet GPIIIa49-66 capable of inducing oxidative platelet fragmentation in the absence of complement. HIV-1-seropositive drug abusers are more prone to develop immune thrombocytopenia (HIV-1-ITP) than non-drug abusers and have a higher coinfection with Hepatitis C virus than non-drug abusers (90% vs 30%). Molecular mimickry with a Hepatitis C protein was sought by screening a phage peptide library with anti-GPIIIa49-66 antibody as bait for peptides sharing homology sequences with HCV protein. Several phage peptide clones had 70% homology with HCV protein. Sera from dually infected thrombocytopenic patients with HCV and HIV-ITP reacted strongly with 4 non-conserved peptides from HCV core-envelope 1. Reactivity correlated inversely with platelet count, r2=0.7, p<0.01. Ab raised against peptide PHC09 in GPIIIa-/- mice induced severe thrombocytopenia in wild-type mice. Affinity-purified IgG against PHC09 induced oxidative platelet fragmentation in vitro. Drug abusers dually infected with HCV and HIV-1 had a greater incidence and severity of thrombocytopenia as well as greater incidence and titer of anti-GPIIIa49-66/PHC09 Ab. NZB/W F1 mice injected with recombinant core envelope 1 developed Ab vs PHC09 and significantly decreased their platelet count, p<0.001. Thus, HCV core envelope 1 can induce thrombocytopenia by molecular mimicry with GPIIIa49-66. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Molecular mimicry and immune thrombocytopenia Richard H. Aster Blood 2009 113: 3887-3888. [Full Text] [PDF] This article has been cited by other articles: D. B. Cines, J. B. Bussel, H. A. Liebman, and E. T. Luning Prak The ITP syndrome: pathogenic and clinical diversity Blood, June 25, 2009; 113(26): 6511 - 6521. [Abstract] [Full Text] [PDF] Z. Li, M. A. Nardi, Y.-S. Li, W. Zhang, R. Pan, S. Dang, H. Yee, D. Quartermain, S. Jonas, and S. Karpatkin C-terminal ADAMTS-18 fragment induces oxidative platelet fragmentation, dissolves platelet aggregates, and protects against carotid artery occlusion and cerebral stroke Blood, June 11, 2009; 113(24): 6051 - 6060. [Abstract] [Full Text] [PDF] R. H. Aster Molecular mimicry and immune thrombocytopenia Blood, April 23, 2009; 113(17): 3887 - 3888. [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020