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Blood, 25 June 2009, Vol. 113, No. 26, pp. 6726-6736. Prepublished online as a Blood First Edition Paper on March 9, 2009; DOI 10.1182/blood-2008-10-184556. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2008-10-184556v1 113/26/6726    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Horst, A. K. Articles by Ritter, U. Search for Related Content PubMed PubMed Citation Articles by Horst, A. K. Articles by Ritter, U. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 21, 2008 Accepted March 2, 2009 CEACAM1+ myeloid cells control angiogenesis in inflammation Andrea K. Horst*, Thomas Bickert, Nancy Brewig, Peter Ludewig, Nico van Rooijen, Udo Schumacher, Nicole Beauchemin, Wulf D. Ito, Bernhard Fleischer, Christoph Wagener, and Uwe Ritter Institute of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, Hamburg, Germany Department of Immunology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany Vrije Universiteit, VUMC, Department of Molecular Cell Biology, Faculty of Medicine, Amsterdam, Netherlands Department of Anatomy II: Experimental Morphology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany McGill Cancer Centre, McGill University, Montreal, QC, Canada University of Ulm, Department of Internal Medicine II, Ulm, Germany Department of Immunology, University of Regensburg, Regensburg, Germany * Corresponding author; email: ahorst{at}uke.uni-hamburg.de. Local inflammation during cutaneous leishmaniasis is accompanied by accumulation of CD11b+ cells at the site of the infection. A functional role for these monocytic cells in local angiogenesis in leishmaniasis has not been described so far. Here, we show that CD11b+ cells express high levels of the myeloid differentiation antigen carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). In experimental cutaneous leishmaniasis in C57BL/6 wild type (B6.WT) and B6.Ceacam1-/- mice, we found that only B6.Ceacam1-/- mice develop oedemas and exhibit impairment of both hem- and lymphangiogenesis. Since CEACAM1 expression correlates with functional angiogenesis, we further analyzed the role of the CD11b+ population. In B6.Ceacam1-/- mice, we found systemic reduction of Ly-6Chigh/CD11bhigh monocyte precursors. To investigate whether CEACAM1+ myeloid cells are causally related to efficient angiogenesis, we used reverse bone marrow transplants (BMT) to restore CEACAM1+ or CEACAM1- bone marrow in B6.Ceacam1-/- or B6.WT recipients, respectively. We found that angiogenesis was restored by CEACAM1+ BMT only. In addition, we observed reduced morphogenic potential of inflammatory cells in Matrigel implants in CEACAM1- backgrounds or after systemic depletion of CD11bhigh macrophages. Taken together, we show for the first time that CEACAM1+ myeloid cells are crucial for angiogenesis in inflammation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Myeloid cell–induced angiogenesis: a sticky business Anna M. Randi and Benedetta Bussolati Blood 2009 113: 6508-6510. [Full Text] [PDF] This article has been cited by other articles: A. M. Randi and B. Bussolati Myeloid cell-induced angiogenesis: a sticky business Blood, June 25, 2009; 113(26): 6508 - 6510. [Full Text] [PDF]

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