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Blood, 4 June 2009, Vol. 113, No. 23, pp. 5920-5926. Prepublished online as a Blood First Edition Paper on February 2, 2009; DOI 10.1182/blood-2008-11-189688. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-11-189688v1 113/23/5920    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jones, R. J. Articles by Matsui, W. Search for Related Content PubMed PubMed Citation Articles by Jones, R. J. Articles by Matsui, W. Related Collections Related Articles in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 17, 2008 Accepted January 24, 2009 Circulating clonotypic B cells in classical Hodgkin's lymphoma Richard J. Jones*, Christopher D. Gocke, Yvette L. Kasamon, Carole B. Miller, Brandy Perkins, James P. Barber, Milada S. Vala, Jonathan M. Gerber, Lan L. Gellert, Mark Siedner, M. Victor Lemas, Sarah Brennan, Richard F. Ambinder, and William Matsui Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins Medical Institutions, Baltimore, MD, United States * Corresponding author; email: rjjones{at}jhmi.edu. Although Hodgkin's and Reed-Sternberg (HRS) cells are B lymphoid cells, they are unlike any normal cells of that lineage. Moreover, the limited proliferative potential of HRS cells belies the clinical aggressiveness of Hodgkin's lymphoma (HL). More than 20 years ago, the L428 HL cell line was reported to contain a small population of phenotypic B cells that appeared responsible for the continued generation of HRS cells. However, this observation has never been corroborated, and such clonotypic B cells have never been documented in HL patients. We found that both the L428 and KM-H2 HL cell lines contained rare B cell subpopulations responsible for the generation and maintenance of the predominant HRS cell population. The B cells within the HL cell lines expressed immunoglobulin light chain, the memory B cell antigen CD27, and the stem cell marker aldehyde dehydrogenase (ALDH). Clonal CD27+ALDHhigh B cells, sharing immunoglobulin gene rearrangements with lymph node HRS cells, were also detected in the blood of most newly-diagnosed HL patients regardless of stage. Although the clinical significance of circulating clonotypic B cells in HL remains unclear, these data suggest they may be the initiating cells for HL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Articles in Blood Online: Clonotypic B cells in classic Hodgkin lymphoma Ralf Küppers Blood 2009 114: 3970-3971. [Full Text] [PDF] Response: Hodgkin lymphoma stem cells Richard J. Jones, Christopher D. Gocke, Richard F. Ambinder, and William Matsui Blood 2009 114: 3971-3972. [Full Text] [PDF] Stem cells in Hodgkin lymphoma? Randy D. Gascoyne Blood 2009 113: 5694. [Full Text] [PDF] This article has been cited by other articles: R. Kuppers Clonotypic B cells in classic Hodgkin lymphoma Blood, October 29, 2009; 114(18): 3970 - 3971. [Full Text] [PDF] R. J. Jones, C. D. Gocke, R. F. Ambinder, and W. Matsui Response: Hodgkin lymphoma stem cells Blood, October 29, 2009; 114(18): 3971 - 3972. [Full Text] [PDF] R. D. Gascoyne Stem cells in Hodgkin lymphoma? Blood, June 4, 2009; 113(23): 5694 - 5694. [Full Text] [PDF]

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