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Blood, 9 July 2009, Vol. 114, No. 2, pp. 328-337. Prepublished online as a Blood First Edition Paper on May 5, 2009; DOI 10.1182/blood-2008-12-192203. This Article Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2008-12-192203v1 114/2/328    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Di Mascio, M. Articles by Lane, H. C. Search for Related Content PubMed PubMed Citation Articles by Di Mascio, M. Articles by Lane, H. C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 5, 2008 Accepted April 22, 2009 Non-invasive in vivo imaging of CD4 cells in SHIV infected non-human primates Michele Di Mascio*, Chang H. Paik, Jorge A. Carrasquillo, Jin-Soo Maeng, Beom-Su Jang, In Soo Shin, Sharat Srinivasula, Russ Byrum, Achilles Neria, William Kopp, Marta Catalfamo, Yoshiaki Nishimura, Keith Reimann, Malcolm Martin, and H. Clifford Lane National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD, United States Memorial Sloan Kettering Cancer Center, New York, NY, United States Biostatistics Research Branch, SAIC Frederick, Inc., NCI-Frederick, Frederick, MD, United States Bioqual, Rockville, MD, United States Clinical Services Program, SAIC Frederick, Inc., NCI-Frederick, Frederick, MD, United States Beth Israel Deaconess Medical Center, Boston, MA, United States * Corresponding author; email: mdimascio{at}niaid.nih.gov. Since the earliest days of the HIV epidemic, the number of CD4+ T cells per unit volume of blood has been recognized as a major prognostic factor for the development of AIDS in persons with HIV infection. It has also been generally accepted that approximately 2% of total body lymphocytes circulate in the blood. In the present study we have utilized a non-depleting humanized anti-CD4 monoclonal antibody labeled with the gamma emitter 111Indium to visualize the CD4+ T cell pool in vivo in non-human primates with SHIV infection. A strong correlation was noted between radiotracer uptake in spleen, tonsil, axillary lymph nodes and peripheral blood CD4 T cell counts (p = 0.75, 0.93 and 0.85, respectively, P < 0.005). The relationship between radiotracer retention in lymphoid tissues and CD4+ T cell counts in the circulation was governed by an exponential law. These data provide an estimate for the total number of lymphocytes in the body as being between 1.9 and 2.9x1012 and suggest that the partition between peripheral blood and lymphoid tissue is between 0.3% and 0.5%. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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