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Blood, 9 July 2009, Vol. 114, No. 2, pp. 290-298. Prepublished online as a Blood First Edition Paper on April 8, 2009; DOI 10.1182/blood-2008-12-195644. This Article Full Text (PDF) Supplemental Methods, Figures, and Videos All Versions of this Article: blood-2008-12-195644v1 114/2/290    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kohler, A. Articles by Geiger, H. Search for Related Content PubMed PubMed Citation Articles by Kohler, A. Articles by Geiger, H. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 24, 2008 Accepted March 22, 2009 Altered cellular dynamics and endosteal location of aged early hematopoietic progenitor cells revealed by time-lapse intravital imaging in long bones Anja Kohler, Vince Schmithorst, Marie-Dominique Filippi, Marnie A. Ryan, Deidre Daria, Matthias Gunzer, and Hartmut Geiger* Institute of Molecular and Clinical Immunology, Otto von Guericke University, Magdeburg, Germany Imaging Research Center, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States Department of Dermatology and Allergic Diseases, Aging Research, University of Ulm, Ulm, Germany * Corresponding author; email: hartmut.geiger{at}uni-ulm.de. Aged hematopoietic stem cells (HSCs) are impaired in supporting hematopoiesis. The molecular and cellular mechanisms of stem cell aging are not well defined. HSCs interact with non-hematopoietic stroma cells in the bone marrow forming the niche. Interactions of hematopoietic cells with the stroma/microenvironment inside bone cavities are central to hematopoiesis as they regulate cell proliferation, self-renewal and differentiation. We recently hypothesized that one underlying cause of altered hematopoiesis in aging might be due to altered interactions of aged stem cells with the microenvironment/niche. We developed time-lapse 2-photon microscopy and novel image analysis algorithms to quantify the dynamics of young and aged hematopoietic cells inside the marrow of long bones of mice in vivo. We report here that aged early hematopoietic progenitor cells (eHPCs) present with increased cell protrusion movement in vivo and localize more distantly to the endosteum compared to young eHPCs. This correlated with reduced adhesion to stroma cells as well as reduced cell-polarity upon adhesion of aged eHPCs. These data support a role of altered eHPC dynamics and altered cell polarity and thus altered niche biology in mechanisms of mammalian aging. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Visualize eHPCs in different zones Tong Yin, Yucai Xie, and Linheng Li Blood 2009 114: 230-231. [Full Text] [PDF] This article has been cited by other articles: Y. Jiang, H. Bonig, T. Ulyanova, K. Chang, and T. Papayannopoulou On the adaptation of endosteal stem cell niche function in response to stress Blood, October 29, 2009; 114(18): 3773 - 3782. [Abstract] [Full Text] [PDF] T. Yin, Y. Xie, and L. Li Visualize eHPCs in different zones Blood, July 9, 2009; 114(2): 230 - 231. [Full Text] [PDF]

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