SEARCH:   Advanced

Blood, 9 April 2009, Vol. 113, No. 15, pp. 3472-3474. Prepublished online as a Blood First Edition Paper on February 6, 2009; DOI 10.1182/blood-2008-12-195677. This Article Full Text (PDF) All Versions of this Article: blood-2008-12-195677v1 113/15/3472    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Busque, L. Articles by Gilliland, D. G. Search for Related Content PubMed PubMed Citation Articles by Busque, L. Articles by Gilliland, D. G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 19, 2008 Accepted February 2, 2009 Skewing of X-inactivation ratios in blood cells of aging women is confirmed by independent methodologies Lambert Busque*, Yves Paquette, Sylvie Provost, Denis-Claude Roy, Ross L. Levine, Luigina Mollica, and D. Gary Gilliland Research Centre, Maisonneuve-Rosemont Hospital, Montreal, Canada Montreal Heart Institute, Montreal, Canada Division of Hematology, Maisonneuve-Rosemont Hospital, Montreal, Canada Leukemia Service, Memorial Sloan Kettering Cancer Center, New York, NY, United States University of Montreal, Montreal, Canada Division of Hematology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States * Corresponding author; email: lbusque.hmr{at}ssss.gouv.qc.ca. Non-random X chromosome inactivation (XCI), also known as skewing, has been documented in the blood cells of a significant proportion of normal aging women using methylation-based assays at the polymorphic human androgen receptor locus (HUMARA). Recent data obtained with a new transcription-based XCI determination method, termed "suppressive PCR", has shed controversy over the validity of XCI ratio results obtained with HUMARA. To resolve this disparity, we analyzed XCI in polymorphonuclear leukocytes of a large cohort of women aged 43 to 100 using HUMARA (n=100), a Taqman SNP assay (n=90), and the suppressive PCR assay (n=67). The three methods yielded similar skewing incidences (42%, 38%, and 40%, respectively), and highly concordant XCI ratios. This confirms that the skewing of XCI ratio seen in blood cells of aging women is a bona fide and robust biological phenomenon. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Agarwal, R. H. Nussenzveig, S. I. Swierczek, C. J. Parker, and J. T. Prchal Does HUMARA assay for assessment of clonal hematopoiesis have shortcomings? Blood, September 10, 2009; 114(11): 2357 - 2358. [Full Text] [PDF] L. Busque, Y. Paquette, L. Mollica, D.-C. Roy, R. L. Levine, and D. G. Gilliland Response: To be skewed or not to be? This is no longer the question Blood, September 10, 2009; 114(11): 2358 - 2359. [Full Text] [PDF]

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020