SEARCH:   Advanced

Blood, 2 July 2009, Vol. 114, No. 1, pp. 49-59. Prepublished online as a Blood First Edition Paper on May 5, 2009; DOI 10.1182/blood-2009-01-197988. This Article Full Text (PDF) All Versions of this Article: blood-2009-01-197988v1 114/1/49    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Grassinger, J. Articles by Nilsson, S. K Search for Related Content PubMed PubMed Citation Articles by Grassinger, J. Articles by Nilsson, S. K Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted January 5, 2009 Accepted April 10, 2009 Thrombin cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with 91 and 41 integrins Jochen Grassinger, David N Haylock, Melonie J Storan, Gemma O Haines, Brenda Williams, Genevieve A Whitty, Andrew R Vinson, Cheang Ly Be, Songhui Li, Esben S Sorensen, Patrick PL Tam, David T Denhardt, Dean Sheppard, Peter F Choong, and Susan K Nilsson* Australian Stem Cell Centre, Clayton, Victoria, Australia Department of Molecular Biology, University of Aarhus, Aarhus, Denmark Embryology Unit, Children's Medical Research Institute, University of Sydney, Westmead, New South Wales, Australia Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, United States University of California San Francisco, San Francisco, CA, United States Department of Orthopaedics and University of Melbourne Department of Surgery, St. Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia Department of Pathology, University of Melbourne, Parkville, Victoria, Australia * Corresponding author; email: susie.nilsson{at}stemcellcentre.edu.au. Osteopontin (OPN), a multifunctional acidic glycoprotein, expressed by osteoblasts within the endosteal region of the bone marrow (BM) suppresses the proliferation of hemopoietic stem and progenitor cells and also regulates their lodgment within the BM after transplantation. Herein we demonstrate that OPN cleavage fragments are the most abundant forms of this protein within the BM. Studies aimed to determine how hemopoietic stem cells (HSCs) interact with OPN revealed for the first time that murine and human HSCs express 91 integrin. The N-terminal thrombin cleavage fragment of OPN through its binding to the 91 and 41 integrins plays a key role in the attraction, retention, regulation and release of hemopoietic stem and progenitor cells to, in and from their BM niche. Thrombin cleaved OPN (trOPN) acts as a chemoattractant for stem and progenitor cells, mediating their migration in a manner that involves interaction with 91 and 41 integrins. In addition, in the absence of OPN there is an increased number of white blood cells and specifically stem and progenitor cells in the peripheral circulation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. C. Forsberg and S. Smith-Berdan Parsing the niche code: the molecular mechanisms governing hematopoietic stem cell adhesion and differentiation Haematologica, November 1, 2009; 94(11): 1477 - 1481. [Full Text] [PDF]

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020