|
|
Blood, 11 June 2009, Vol. 113, No. 24, pp. 6237-6245.
Prepublished online as a Blood First Edition Paper on April 15, 2009; DOI 10.1182/blood-2009-02-205450.
 Previous Article | Next Article 
Submitted February 12, 2009
Accepted April 9, 2009
Imaging of the diffusion of single band 3 molecules on normal and mutant erythrocytes
Gayani C Kodippili, Jeff Spector, Caitlin Sullivan, Frans A. Kuypers, Richard Labotka, Patrick G. Gallagher, Ken Ritchie, and Philip S. Low*
Department of Chemistry, Purdue University, West Lafayette, IN, United States
Department of Physics, Purdue University, West Lafayette, IN, United States
Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, United States
Children's Hospital Oakland, Research Institute, Oakland, CA, United States
Division of Pediatric Hematology/Oncology, University of Illinois, Chicago, IL, United States
Department of Pediatrics, Yale University School of Medicine, New Haven, CT, United States
* Corresponding author; email: plow{at}purdue.edu.
Membrane-spanning proteins may interact with a variety of other integral and peripheral membrane proteins via a diversity of protein-protein interactions. Not surprisingly, defects or mutations in any one of these interacting components can impact the physical and biological properties on the entire complex. Here we use quantum dots to image the diffusion of individual band 3 molecules in the plasma membranes of intact human erythrocytes from healthy volunteers and patients with defects in one of their membrane components, leading to well known red cell pathologies (hereditary spherocytosis, hereditary elliptocytosis, hereditary hydrocytosis, southeast asian ovalocytosis, and hereditary pyropoikilocytosis). After characterizing the motile properties of the major sub-populations of band 3 in intact normal erythrocytes, we demonstrate that the properties of these sub-populations of band 3 change significantly in diseased cells, as evidenced by changes in the microscopic and macroscopic diffusion coefficients of band 3 and in the compartment sizes in which the different band 3 populations can diffuse. Because the above membrane abnormalities largely arise from defects in other membrane components (e.g. spectrin, ankyrin, etc.), these data suggest that single particle tracking of band 3 might constitute a useful tool for characterizing the general structural integrity of the human erythrocyte membrane.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
|
|
