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Blood, 10 September 2009, Vol. 114, No. 11, pp. 2315-2322. Prepublished online as a Blood First Edition Paper on June 12, 2009; DOI 10.1182/blood-2009-04-214387. This Article Full Text (PDF) All Versions of this Article: blood-2009-04-214387v1 114/11/2315    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Google Scholar Articles by Desmarets, M. Articles by Zimring, J. C. PubMed PubMed Citation Articles by Desmarets, M. Articles by Zimring, J. C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 2, 2009 Accepted June 3, 2009 Minor histocompatibility antigens on transfused leukoreduced units of red blood cells induce bone marrow transplant rejection in a mouse model Maxime Desmarets, Chantel M. Cadwell, Kenneth R. Peterson, Renee Neades, and James C. Zimring* Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, United States Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, United States * Corresponding author; email: jzimrin{at}emory.edu. When successful, HLA matched bone marrow transplantation (BMT) with reduced intensity conditioning is a cure for several non-malignant hematological disorders that require chronic transfusion, such as sickle cell disease and aplastic anemia. However, there are unusually high BMT rejection rates in these patients. Rejection correlates with the number of pre-BMT transfusions, and it has been hypothesized that pre-immunization to antigens on transfused blood may prime BMT rejection. Using a novel mouse model of RBC transfusion and MHC matched BMT, we report that transfusion of RBC products induced BMT rejection across minor histocompatibility antigen (mHA) barriers. It has been proposed that contaminating leukocytes are responsible for transfusion-induced BMT rejection; however, filter leukoreduction did not prevent rejection in the current studies. Moreover, we generated a novel transgenic mouse with RBC specific expression of a model mHA, and demonstrated that transfusion of RBCs induced a CD8+ T cell response. Together, these data suggest that mHAs on RBCs themselves are capable of inducing BMT rejection. Cellular immunization to mHAs is neither monitored nor managed by current transfusion medicine practice; however, the current data suggest that mHAs on RBCs may represent an unappreciated and significant consequence of RBC transfusion. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: RBC transfusion and BMT rejection Allen R. Chen Blood 2009 114: 2209-2210. [Full Text] [PDF] This article has been cited by other articles: A. R. Chen RBC transfusion and BMT rejection Blood, September 10, 2009; 114(11): 2209 - 2210. [Full Text] [PDF]

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