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Blood First Edition Paper, prepublished online November 6, 2009; DOI 10.1182/blood-2009-07-228106. This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Ito, C. Y. Articles by Zandstra, P. W. PubMed PubMed Citation Articles by Ito, C. Y. Articles by Zandstra, P. W. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 9, 2009; accepted October 10, 2009. The AC133+CD38-, but not the rhodamine-low phenotype, tracks LTC-IC and SRC function in human cord blood ex vivo expansion cultures Caryn Y. Ito1, Daniel C. Kirouac1, Gerard J. Madlambayan2, Mei Yu1, Ian Rogers3 and Peter W. Zandstra4,4 1 Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada; 2 Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Gainesville, FL, United States; 3 Mount Sinai Hospital Samuel Lunenfeld Research Institute, Toronto, Canada; 4 Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada * Corresponding author; email: peter.zandstra{at}utoronto.ca Abstract Phenotypic markers associated with human hematopoietic stem cells (HSC) were developed and validated using uncultured cells. Since phenotype and function can be dissociated during culture, better markers to prospectively track and isolate HSC in ex vivo cultures would be instrumental in advancing HSC-based therapies. Using an expansion system previously shown to increase hematopoietic progenitors and SCID-repopulating cells (SRC), we demonstrated that the Rhodamine-low phenotype was lost, while AC133 expression was retained throughout culture. Furthermore, the AC133+CD38- subpopulation was significantly enriched in long term culture-initiating cells (LTC-IC) and SRC post-culture. Pre- and post-culture analysis of total nucleated cell number, LTC-IC and limiting dilution analysis in NOD/SCID mice, showed a 43-fold expansion of the AC133+CD38- subpopulation that corresponded with a 7.3 and 4.4-fold expansion of LTC-IC and SRC in this subpopulation, respectively. Thus, AC133+CD38- is an improved marker that tracks and enriches for LTC-IC and SRC in ex vivo cultures. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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