http://bloodjournal.hematologylibrary.org Blood RSS feed -- recent TRANSFUSION MEDICINE articles 1528-0020 Blood 0006-4971 http://bloodjournal.hematologylibrary.org/icons/banner/title.gif http://bloodjournal.hematologylibrary.org http://bloodjournal.hematologylibrary.org/cgi/content/short/111/7/3880?rss=1 Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n = 30) and without MC (n = 29), for 4 functionally significant SNPs: TNF (–308), IL 10 (–1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (–308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

]]> 2008-03-24 info:doi/10.1182/blood-2007-08-107144 American Society of Hematology 7 111 3883 2008-04-01 3880 TRANSFUSION MEDICINE